James R Springstead, PhD
Assistant Professor, Program in Medical Engineering
1000 Oakland Drive
Kalamazoo, MI 49008
James Springstead, PhD, received his bachelor's degree in chemical engineering from the University of Pennsylvania in 2001, and then earned his PhD in chemical engineering at the University of California, Los Angeles in 2008, under the direction of Harold Monbouquette. During his studies at UCLA, Dr. Springstead discovered and characterized a novel trifunctional polypeptide and characterized the membrane lipid of the hypethermophilic archaeon, Archaeoglobus fulgidus, using mass spectrometry. Furthermore, he developed a method to measure the thermal area expansivity of lipid vesicles with multi angle laser light scattering, applicable as a quality control method for liposomes used in drug delivery and other industries. During his time at UCLA he also taught classes in chemical engineering and molecular biotechnology and held leadership positions in several UCLA organizations.
Immediately following his PhD studies, Dr. Springstead joined the prolific Atherosclerosis Research Unit in the Cardiology Division of the UCLA Department of Medicine as a postdoctoral researcher, his present position, performing research under the direction of Dr. Andrew Watson and Dr. Judith Berliner. In this work, he applies his enthusiasm and expertise in studying lipid chemistry to solving biological problems and studying lipids involved in medicine. He specifically uses mass spectrometry, western blotting, and microarray analysis to study the activation of endothelial cells by oxidized phospholipids, an event which can lead to the development of atherosclerosis, an underlying condition in heart disease and stroke. In his future research, he will further elucidate the mechanisms involved in atherosclerosis, and he will also explore the development and delivery of novel therapeutics for treatment of atherosclerosis.
Education and Training
- Postdoctoral Fellowship 2012, Vascular Biology Training Program, University of California-Los Angeles
- PhD 2008, Chemical Engineering, University of California-Los Angeles
- MS 2005, Chemical Engineering, University of California-Los Angeles
- BS 2001, Chemical Engineering, University of Pennsylvania
- Chemical Engineering
Di Gioia M., Spreafico R., Springstead J.R., Mendelson M.M., Joehanes R., Levy D., Zanoni I. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation Nature Immunology. 2020;21:42-53. doi: 10.1038/s41590-019-0539-2.
Awedat K., Abdel-Qader I., Springstead J. Prostate cancer recognition based on mass spectrometry sensing data and data fingerprint recovery Biomedical Signal Processing and Control. 2017;33:392-399. doi: 10.1016/j.bspc.2016.12.003.
Awedat K., Alajmi M., Springstead J. Compressive sensing of jointly sparse signals as a method for dimensionality reduction of mass spectrometry data IEEE International Conference on Electro Information Technology. 2017:80-85. doi: 10.1109/EIT.2017.8053334.
Hogan N., Whalen M., Stolze L., Hadeli N., Lam M., Springstead J., Glass C., Romanoski C. Transcriptional networks specifying homeostatic and inflammatory programs of gene expression in human aortic endothelial cells eLife. 2017;6 doi: 10.7554/eLife.22536.
Maabreh M., Qolomany B., Gupta A., Springstead J. Optimizing protein search engines using particle swarm optimization Proceedings - 2017 IEEE 17th International Conference on Bioinformatics and Bioengineering, BIBE 2017. 2017;2018-January:340-345. doi: 10.1109/BIBE.2017.00-31.
Maabreh M., Qolomany B., Springstead J., Alsmadi I., Gupta A. Deep vs. shallow learning-based filters of MS/MS spectra in support of protein search engines Proceedings - 2017 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2017. 2017;2017-January:1175-1182. doi: 10.1109/BIBM.2017.8217824.
Zanoni I., Tan Y., Di Gioia M., Springstead J., Kagan J. By Capturing Inflammatory Lipids Released from Dying Cells, the Receptor CD14 Induces Inflammasome-Dependent Phagocyte Hyperactivation Immunity. 2017;47:697-709.e3. doi: 10.1016/j.immuni.2017.09.010.
Awedat K., Abdel-Qader I., Springstead J. Mass spectrometry sensing data for robust cancer classification IEEE International Conference on Electro Information Technology. 2016;2016-August:258-262. doi: 10.1109/EIT.2016.7535250.
Awedat K., Alajmi M., Springstead J.R. Compressive sensing based for mass spectrometry reconstruction Proceedings of the IEEE National Aerospace Electronics Conference, NAECON. 2016:314-317. doi: 10.1109/NAECON.2016.7856821.
Springstead J., AbuBakr S. Global engagement strategies for engineering students at Western Michigan University Liaison Functions 2016 - Core Programming Area at the 2016 AIChE Annual Meeting. 2016:248-249.
Zanoni I., Tan Y., Gioia M.D., Broggi A., Ruan J., Shi J., Donado C., Shao F., Wu H., Springstead J.R., Kagan J.C. An endogenous caspase-11 ligand elicits interleukin-1 release from living dendritic cells Science. 2016;352:1232-1236. doi: 10.1126/science.aaf3036.
Emert B., Hasin-Brumshtein Y., Springstead J.R., Vakili L., Berliner J.A., Lusis A.J. HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms Journal of Lipid Research. 2014;55:1678-1692. doi: 10.1194/jlr.M047738.
Chattopadhyay A., Navab M., Hough G., Gao F., Meriwether D., Grijalva V., Springstead J.R., Palgnachari M.N., Namiri-Kalantari R., Su F., Van Lenten B.J., Wagner A.C., Anantharamaiah G.M., Farias-Eisener R., Reddy S.T., Fogelman A.M. A novel approach to oral apoA-I mimetic therapy Journal of Lipid Research. 2013;54:995-1010. doi: 10.1194/jlr.M033555.
Zhong W., Springstead J., Al-Mubarak R., Lee S., Li R., Emert B., Berliner J., Jung M. An epoxyisoprostane is a major regulator of endothelial cell function Journal of Medicinal Chemistry. 2013;56:8521-8532. doi: 10.1021/jm400959q.
Lee S., Birukov K., Romanoski C., Springstead J., Lusis A., Berliner J. Role of phospholipid oxidation products in atherosclerosis Circulation Research. 2012;111:778-799. doi: 10.1161/CIRCRESAHA.111.256859.
Lee S., Springstead J.R., Parks B.W., Romanoski C.E., Palvolgyi R., Ho T., Nguyen P., Lusis A.J., Berliner J.A. Metalloproteinase processing of HBEGF is a proximal event in the response of human aortic endothelial cells to oxidized phospholipids Arteriosclerosis, Thrombosis, and Vascular Biology. 2012;32:1246-1254. doi: 10.1161/ATVBAHA.111.241257.
Springstead J.R., Gugiu B.G., Lee S., Seung C., Watson A.D., Berliner J.A. Evidence for the importance of OxPAPC interaction with cysteines in regulating endothelial cell function Journal of Lipid Research. 2012;53:1304-1315. doi: 10.1194/jlr.M025320.
Lim S., Springstead J., Yu M., Bartkowski W., Schröder I., Monbouquette H. Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus Extremophiles. 2009;13:191-198. doi: 10.1007/s00792-008-0209-z.
Lai D., Springstead J., Monbouquette H. Effect of growth temperature on ether lipid biochemistry in Archaeoglobus fulgidus Extremophiles. 2008;12:271-278. doi: 10.1007/s00792-007-0126-6.