The Department of Investigative Medicine provides ample opportunities for student and residents to participate with faculty in research and scholarly activities. We offer experiences that enable discovery and promote creativity. As a department, we are committed to pursuing inquiry, disseminating knowledge, and fostering critical thinking that encourages lifelong learning.
Research and Scholarly Activity, 2019-22
Recent Publications
Webster S.E., Tsuji N.L., Clemente M.J., Holodick N.E. Age-related changes in antigen-specific natural antibodies are influenced by sex Frontiers in Immunology. 2023;13
Gutknecht M.F., Kaku H., Rothstein T.L. Microparticle immunocapture assay for quantitation of protein multimer amount and size Cell Reports Methods. 2022;2
Gutknecht M.F., Kaku H., Rothstein T.L. Microparticle immunocapture assay for quantitation of protein multimer amount and size Cell Reports Methods. 2022;2
Husain-Krautter S., Lee J., Vos D., Gallego J.A., Malhotra A.K., Rothstein T.L. Skewing of the antibody repertoire in cerebrospinal fluid B cells from healthy controls and patients with schizophrenia Behavioural Brain Research. 2022;422
Jondle C.N., Sylvester P.A., Schmalzriedt D.L., Njoya K., Tarakanova V.L. The Antagonism between the Murine Gammaherpesvirus Protein Kinase and Global Interferon Regulatory Factor 1 Expression Shapes the Establishment of Chronic Infection Journal of Virology. 2022;96
Jondle C.N., Tarakanova V.L. T Cell-Intrinsic Interleukin 17 Receptor A Signaling Supports the Establishment of Chronic Murine Gammaherpesvirus 68 Infection Journal of Virology. 2022;96
Kaku H., Balaj A.R., Rothstein T.L. Small Heat Shock Proteins Collaborate with FAIM to Prevent Accumulation of Misfolded Protein Aggregates International Journal of Molecular Sciences. 2022;23(19)
Rodriguez-Zhurbenko N., Quach T.D., Rothstein T.L., Hernandez A.M. Human B-1 cells are important contributors to the naturally-occurring IgM pool against the tumor-associated ganglioside Neu5GcGM3 Frontiers in Immunology. 2022;13
Srikakulapu P., Pattarabanjird T., Upadhye A., Bontha S.V., Osinski V., Marshall M.A., Garmey J., Deroissart J., Prohaska T.A., Witztum J.L., Binder C.J., Holodick N.E., Rothstein T.L., McNamara C.A. B-1b Cells Have Unique Functional Traits Compared to B-1a Cells at Homeostasis and in Aged Hyperlipidemic Mice With Atherosclerosis Frontiers in Immunology. 2022;13
Sylvester P.A., Jondle C.N., Schmalzriedt D.L., Dittel B.N., Tarakanova V.L. T Cell-Specific STAT1 Expression Promotes Lytic Replication and Supports the Establishment of Gammaherpesvirus Latent Reservoir in Splenic B Cells mBio. 2022;13
Webster S.E., Ryali B., Clemente M.J., Tsuji N.L., Holodick N.E. Sex Influences Age-Related Changes in Natural Antibodies and CD5<sup>+</sup> B-1 Cells Journal of Immunology. 2022;208:1755-1771.
Johnson K.E., Sylvester P.A., Jondle C.N., Aurubin C.A., Tarakanovaa V.L. Interferon Regulatory Factor 3 Supports the Establishment of Chronic Gammaherpesvirus Infection in a Route- And Dose-Dependent Manner Journal of Virology. 2021;95(9)
Jondle C.N., Johnson K.E., Aurubin C., Sylvester P., Xin G., Cui W., Huppler A.R., Tarakanova V.L. Gammaherpesvirus usurps host il-17 signaling to support the establishment of chronic infection mBio. 2021;12(2)
Jondle C.N., Johnson K.E., Mboko W.P., Tarakanova V.L. T cell-intrinsic interferon regulatory factor 1 expression suppresses differentiation of CD4<sup>+</sup> T cell populations that support chronic gammaherpesvirus infection Journal of Virology. 2021;95(20)
Kaku H., Ludlow A.V., Gutknecht M.F., Rothstein T.L. Fas Apoptosis Inhibitory Molecule Blocks and Dissolves Pathological Amyloid-β Species Frontiers in Molecular Neuroscience. 2021;14
Kaku H., Ludlow A.V., Gutknecht M.F., Rothstein T.L. Fas Apoptosis Inhibitory Molecule Blocks and Dissolves Pathological Amyloid-β Species Frontiers in Molecular Neuroscience. 2021;14
Khan N., Hu Y., Lowell C.A., Rothstein T.L. Signal integration by translocation and phosphorylation of PKCd in the B Cell alternate pathway Journal of Immunology. 2021;207:2288-2296.
Khan N., Rothstein T.L. The Alternate Pathway for BCR Signaling Induced by IL-4 Requires Lyn Tyrosine Kinase Journal of Molecular Biology. 2021;433
Ludlow A., George N., Glassford M., Udenberg K., Hannibal M.C., Schwalm C., Scott K., Rothstein T.L., Singh S.A. The Use of B-Cell Polysome Profiling to Validate Novel RPL5 (uL18) and RPL26 (uL24) Variants in Diamond-Blackfan Anemia Journal of Pediatric Hematology/Oncology. 2021;43:e336-e340.
Sylvester P.A., Jondle C.N., Stoltz K.P., Lanham J., Dittel B.N., Tarakanova V.L. Conserved gammaherpesvirus protein kinase counters the antiviral effects of myeloid cell-specific stat1 expression to promote the establishment of splenic b cell latency Journal of Virology. 2021;95(17)
Yu L., Lemay P., Ludlow A., Guyot M.C., Jones M., Mohamed F.F., Saroya G.A., Panaretos C., Schneider E., Wang Y., Myers G., Khoriaty R., Li Q., Franceschi R., Engel J.D., Kaartinen V., Rothstein T.L., Justice M.J., Kibar Z., Singh S.A. A new murine Rpl5 (uL18) mutation provides a unique model of variably penetrant Diamond-Blackfan anemia Blood Advances. 2021;5:4167-4178.
Johnson K.E., Aurubin C.A., Jondle C.N., Lange P.T., Tarakanova V.L. Interferon regulatory factor 7 attenuates chronic gammaherpesvirus infection Journal of Virology. 2020;94(24)
Johnson K.E., Lange P.T., Jondle C.N., Volberding P.J., Lorenz U.M., Cui W., Dittel B.N., Tarakanova V.L. B cell-intrinsic SHP1 expression promotes the gammaherpesvirus-driven germinal center response and the establishment of chronic infection Journal of Virology. 2020;94(1)
Jondle C.N., Johnson K.E., Uitenbroek A.A., Sylvester P.A., Nguyen C., Cui W., Tarakanova V.L. B cell-intrinsic expression of interferon regulatory factor 1 supports chronic murine gammaherpesvirus 68 infection Journal of Virology. 2020;94(13)
Jondle C.N., Tarakanova V.L. Innate immunity and alpha/gammaherpesviruses: first impressions last a lifetime Current Opinion in Virology. 2020;44:81-89.
Kaku H., Ludlow A.V., Gutknecht M.F., Rothstein T.L. FAIM Opposes Aggregation of Mutant SOD1 That Typifies Some Forms of Familial Amyotrophic Lateral Sclerosis Frontiers in Neuroscience. 2020;14
Kaku H., Ludlow A.V., Gutknecht M.F., Rothstein T.L. FAIM Opposes Aggregation of Mutant SOD1 That Typifies Some Forms of Familial Amyotrophic Lateral Sclerosis Frontiers in Neuroscience. 2020;14
Kaku H., Rothstein T.L. FAIM Is a Non-redundant Defender of Cellular Viability in the Face of Heat and Oxidative Stress and Interferes With Accumulation of Stress-Induced Protein Aggregates Frontiers in Molecular Biosciences. 2020;7
Kaku H., Rothstein T.L. FAIM Is a Non-redundant Defender of Cellular Viability in the Face of Heat and Oxidative Stress and Interferes With Accumulation of Stress-Induced Protein Aggregates Frontiers in Molecular Biosciences. 2020;7
Tsuji N., Rothstein T.L., Holodick N.E. Antigen Receptor Specificity and Cell Location Influence the Diversification and Selection of the B-1a Cell Pool with Age Journal of immunology (Baltimore, Md. : 1950). 2020;205:741-759.
Darrah E., Jondle C., Johnson K., Xin G., Lange P., Cui W., Olteanu H., Tarakanova V. Conserved gammaherpesvirus protein kinase selectively promotes irrelevant B cell responses Journal of Virology. 2019;93(8)
Ghosn E., Dorshkind K., Herzenberg L.A., Holodick N., Kantor A., Montecino-Rodriguez E., Rothstein T.L., Silverman G., Yang Y., Yoder M.C., Yoshimoto M. B1 B cell progenitors Science. 2019;364:248.
Guo B., Ludlow A.V., Brightwell A.S., Rothstein T.L. Impairment of PD-L2 positive B1a cells enhances susceptibility to sepsis in RasGRP1-deficient mice Cellular Immunology. 2019;346
Lange P.T., Jondle C.N., Darrah E.J., Johnson K.E., Tarakanovaa V.L. LXR alpha restricts gammaherpesvirus reactivation from latently infected peritoneal cells Journal of Virology. 2019;93(6)
Rodriguez-Zhurbenko N., Quach T.D., Hopkins T.J., Rothstein T.L., Hernandez A.M. Human B-1 cells and B-1 cell antibodies change with advancing age Frontiers in Immunology. 2019;10
Stoltz K., Jondle C., Pulakanti K., Sylvester P., Urrutia R., Rao S., Tarakanova V. Tumor suppressor Interferon Regulatory Factor 1 selectively blocks expression of endogenous retrovirus Virology. 2019;526:52-60.
Upadhye A., Srikakulapu P., Gonen A., Hendrikx S., Perry H.M., Nguyen A., McSkimming C., Marshall M.A., Garmey J.C., Taylor A.M., Bender T.P., Tsimikas S., Holodick N.E., Rothstein T.L., Witztum J.L., McNamara C.A. Diversification and CXCR4-Dependent Establishment of the Bone Marrow B-1a Cell Pool Governs Atheroprotective IgM Production Linked to Human Coronary Atherosclerosis Circulation research. 2019;125:e55-e70.
Aziz M., Ode Y., Zhou M., Ochani M., Holodick N., Rothstein T., Wang P. B-1a cells protect mice from sepsis-induced acute lung injury Molecular Medicine. 2018;24
Chauhan A., Sun Y., Sukumaran P., Quenum Zangbede F.O., Jondle C.N., Sharma A., Evans D.L., Chauhan P., Szlabick R.E., Aaland M.O., Birnbaumer L., Sharma J., Singh B.B., Mishra B.B. M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry iScience. 2018;8:85-102.
Gallego J., Blanco E., Husain-Krautter S., Madeline Fagen E., Moreno-Merino P., del Ojo-Jiménez J., Ahmed A., Rothstein T., Lencz T., Malhotra A. Cytokines in cerebrospinal fluid of patients with schizophrenia spectrum disorders: New data and an updated meta-analysis Schizophrenia Research. 2018;202:64-71.
Huang W., Quach T.D., Dascalu C., Liu Z., Leung T., Byrne-Steele M., Pan W., Yang Q., Han J., Lesser M., Rothstein T.L., Furie R., Mackay M., Aranow C., Davidson A. Belimumab promotes negative selection of activated autoreactive B cells in systemic lupus erythematosus patients JCI insight. 2018;3
Jondle C., Gupta K., Mishra B., Sharma J. Klebsiella pneumoniae infection of murine neutrophils impairs their efferocytic clearance by modulating cell death machinery PLoS Pathogens. 2018;14(10)
Ward S.E., O’Sullivan J.M., Drakeford C., Aguila S., Jondle C.N., Sharma J., Fallon P.G., Brophy T.M., Preston R.J.S., Smyth P., Sheils O., Chion A., O’Donnell J.S. A novel role for the macrophage galactose-type lectin receptor in mediating von Willebrand factor clearance Blood. 2018;131(8):911-916.
Aziz M., Holodick N., Rothstein T., Wang P. B-1a cells protect mice from sepsis: Critical role of creb Journal of Immunology. 2017;199(2):750-760.
Hernandez A., Holodick N. Editorial: Natural antibodies in health and disease Frontiers in Immunology. 2017;8(DEC)
Holodick N., Rodríguez-Zhurbenko N., Hernández A. Defining natural antibodies Frontiers in Immunology. 2017;8(JUL)
Kaku H., Holodick N., Tumang J., Rothstein T. CD25<sup>+</sup> B-1a cells express Aicda Frontiers in Immunology. 2017;8
Martínez D., Pupo A., Cabrera L., Raymond J., Holodick N., Hernández A. B-CD8<sup>+</sup> T cell interactions in the anti-idiotypic response against a self-antibody Journal of Immunology Research. 2017;2017
Sharma A., Simonson T.J., Jondle C.N., Mishra B.B., Sharma J. Mincle-mediated neutrophil extracellular trap formation by regulation of autophagy Journal of Infectious Diseases. 2017;215(7):1040-1048.
Cui X., Zhang L., Magli A.R., Catera R., Yan X.J., Griffin D.O., Rothstein T.L., Barrientos J., Kolitz J.E., Allen S.L., Rai K.R., Chiorazzi N., Chu C.C. Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability Leukemia. 2016;30(1):74-85.
Guo B., Rothstein T. RasGRP1 is an essential signaling molecule for development of B1a cells with autoantigen receptors Journal of Immunology. 2016;196:2583-2590.
Guo B., Zhang L., Chiorazzi N., Rothstein T.L. IL-4 rescues surface IgM expression in chronic lymphocytic leukemia Blood. 2016;128:553-562.
Holodick N., Vizconde T., Hopkins T., Rothstein T. Age-related decline in natural igm function: Diversification and selection of the B-1a cell pool with age Journal of Immunology. 2016;196(10):4348-4357.
Holodick N.E., Zeumer L., Rothstein T.L., Morel L. Expansion of B-1a cells with germline heavy chain sequence in lupus mice Frontiers in Immunology. 2016;7(MAR)
Jondle C.N., Sharma A., Simonson T.J., Larson B., Mishra B.B., Sharma J. Macrophage galactose-type lectin-1 deficiency is associated with increased neutrophilia and hyperinflammation in gram-negative pneumonia Journal of Immunology. 2016;196(7):3088-3096.
Kramer J.M., Holodick N.E., Vizconde T.C., Raman I., Yan M., Li Q.Z., Gaile D.P., Rothstein T.L. Analysis of IgM antibody production and repertoire in a mouse model of Sjögren’s syndrome Journal of Leukocyte Biology. 2016;99(2):321-331.
Olofsson P., Steinberg B., Sobbi R., Cox M., Ahmed M., Oswald M., Szekeres F., Hanes W., Introini A., Liu S., Holodick N., Rothstein T., Lövdahl C., Chavan S., Yang H., Pavlov V., Broliden K., Andersson U., Diamond B., Miller E., Arner A., Gregersen P., Backx P., Mak T., Tracey K. Blood pressure regulation by CD4<sup>+</sup> lymphocytes expressing choline acetyltransferase Nature Biotechnology. 2016;34(10):1066-1071.
Quach T., Rodriguez-Zhurbenko N., Hopkins T., Guo X., Hernandez A., Li W., Rothstein T. Distinctions among circulating antibody-secreting cell populations, including B-1 cells, in human adult peripheral blood Journal of Immunology. 2016;196(3):1060-1069.
Quách T.D., Hopkins T.J., Holodick N.E., Vuyyuru R., Manser T., Bayer R.L., Rothstein T.L. Human B-1 and B-2 B cells develop from Lin<sup>-</sup>CD34<sup>+</sup>CD38<sup>lo</sup> stem cells Journal of Immunology. 2016;197(10):3950-3958.
Rothstein T. Natural antibodies as rheostats for susceptibility to chronic diseases in the aged Frontiers in Immunology. 2016;7(APR)
Rothstein T., Holodick N. Activation of B-1 Cells Encyclopedia of Immunobiology. 2016;3:237-243.
Sharma A., Kiripolsky J., Klimatcheva E., Howell A., Fereidouni F., Levenson R., Rothstein T.L., Kramer J.M. Early BAFF receptor blockade mitigates murine Sjögren's syndrome: Concomitant targeting of CXCL13 and the BAFF receptor prevents salivary hypofunction Clinical Immunology. 2016;164:85-94.
Aziz M., Holodick N.E., Rothstein T.L., Wang P. The role of B-1 cells in inflammation Immunologic Research. 2015;63(1-3):153-166.
Engelbertsen D., Vallejo J., Dan Quách T., Fredrikson G.N., Alm R., Hedblad B., Björkbacka H., Rothstein T.L., Nilsson J., Bengtsson E. Low levels of IgM antibodies against an advanced glycation endproduct-modified apolipoprotein B100 peptide predict cardiovascular events in nondiabetic subjects Journal of Immunology. 2015;195(7):3020-3025.
Holodick N., Rothstein T. B cells in the aging immune system: Time to consider B-1 cells Annals of the New York Academy of Sciences. 2015;1362(1):176-187.
Husain-Krautter S., Kramer J.M., Li W., Guo B., Rothstein T.L. The osteopontin transgenic mouse is a new model for Sjögren's syndrome Clinical Immunology. 2015;157:30-42.
Rothstein T., Herzenberg L., Holodick N., Ghosn E. B-1 Cell Development and Function Annals of the New York Academy of Sciences. 2015;1362(1):v-vi.
Rothstein T., Quach T. The human counterpart of mouse B-1 cells Annals of the New York Academy of Sciences. 2015;1362(1):143-152.